[dr_medvedev]

Am J Trop Med Hyg. 2004 Dec;71(6):831-5. Related Articles, Links
Detection of Toxoplasma gondii DNA and specific antibodies in high-risk pregnant women.
Nimri L, Pelloux H, Elkhatib L.
Department of Medical Laboratory Sciences, Jordan University of Science and Technology, Irbid, Jordan. [Ссылки доступны только зарегистрированным пользователям ]
Primary maternal infection with toxoplasmosis during pregnancy is frequently associated with transplacental transmission to the fetus. This study was conducted to test the utility of a polymerase chain reaction (PCR) assay to detect recent infections with Toxoplasma in pregnant women. One hundred forty-eight women with high-risk pregnancies who had abnormal pregnancy outcomes (cases) and 100 with normal pregnancies (controls) were tested for the presence of Toxoplasma DNA in their blood by a nested PCR and specific antibodies to Toxoplasma by an enzyme-linked immunosorbent assay. The IgG results of the cases differed significantly from those of the controls (54% and 12%, respectively; P < 0.02). Four (2.7%) of the cases were IgM positive, but none of the controls were positive. Detection of Toxoplasma DNA in 20 (8.1%) of the IgG-positive cases suggests a recent infection. The risk factors associated with the infection were eating raw meat and contact with soil. The diagnostic serology of recent infection in early pregnancy could be confirmed by a positive Toxoplasma-specific PCR result in blood samples collected in the first half of pregnancy, even in the presence of serologic results difficult to interpret due to the lack of sequential follow-up during pregnancy.
PMID: 15642979 [PubMed - indexed for MEDLINE]